Determination of the Role of HA, HYAL and HA- Receptor Expression in A Rat Model of Middle Cerebral Artery Occlusion

Authors

  • Ahmed Al Q* The School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK Author
  • John J G The School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK Author
  • Jerzy K Department of Neurology, Stroke Unit, Institute D’Investigacio Biomedico De Belvitge, Barcelona, Spain Author
  • Mark S The School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK Author

Keywords:

MCAO, rat stroke model, hyaladherins, hyaluronidase1 and 2, RHAMM, CD44

Abstract

Hyaluronan (HA), a component of the extracellular matrix, has been implicated in regulating angiogenesis and cell proliferation, migration, and signaling. We used the rat MCAO to show hyaluronan accumulation in stroke-affected areas. Using RT-PCR and Western blotting we showed up-regulation of hyaluronidase-1 and 2 between 1h and 21 days after stroke. Hyaluronidase-1 was up regulated earlier than hyaluronidase-2. RHAMM and CD44 receptors were also increasedafter stroke. Immunohistochemistry results, showed an association of hyaluronidases1/2 and hyaladherins with neurons in the infarcted and peri-infarcted regions and hyaluronidase-1 with micro vessels. HA synthesis and degradation in the stroke hemisphere might have an impact on neuronal survival, angiogenesis and general tissue remodeling after stroke.

 

 

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Published

2019-04-18

Issue

Vol. 2 No. 1 (2019)

Section

Articles